Dear Wei Zang
have you removed the SDS from your sample? SDS will create problems. In case
you haven't there are solutions to remove SDS prior to separation on RP type
columns. I'm away from my office and answering through a private access. If
you e-mail to the below listed address I can send you literature
information.
Detergents do generate agglomeration of the sample, even in MS. Hence the
have to be removed.
Best regards:
Jean-Pierre
--------------------------------------------------------
Jean-Pierre Salzmann
LC Packings -- A Dionex Company
80 Carolina Street
San Francisco, CA 94103
Phone 415-552-1855
Fax 415-552-1959
Cell phone 415-902-4145
email jps@lcpackingsusa.com
www.lcpackings.com
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> ----------
> From: "Wei Zhang" <WXZhang@genetics.com>
> Date: Tue, 07 Nov 2000 10:22:30 -0500
> To: Recipients of ABRF List <abrf@aecom.yu.edu>
> Subject: RP-HPLC for mAb
>
> Item Type: Task
> Start Date: 11/07/2000 (Tuesday)
> Due: 11/07/2000 (Tuesday)
>
> We are developing RP-HPLC methods for mAbs. We've tried C18, C4, -CN and
> phenyl columns from different vendors, none of them gave good results in
> terms of peak shape and resolution. We saw some minor bands in SDS-PAGE and
> are trying to use RP-HPLC to resolve them. Could you please share with us
> your experience with RP-HPLC of mAbs or other large proteins? Thanks a lot!
>
> Wei Zhang, Ph.D.
> Genetics Institute
> wxzhang@genetics.com
>
>
>
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