Re: RP-HPLC for mAb

From: fperini@unmc.edu
Date: Fri Nov 10 2000 - 17:50:59 EST


I gave you earlier some information about Synchrom columns.One address
which I have is:Micra Scientific,Inc.
1955 Techny Rd,Suite 1;Northbrook.IL 60062.The Web site:www.micrasci.com
 However,the literature which I requested came from Darien,IL
ph.1-800-556-4272

"Wei Zhang" <WXZhang@genetics.com>@aecom.yu.edu> on 11/07/2000 09:22:30 AM

Sent by: Association of Biomolecular Resource Facilities
      <abrf-request@aecom.yu.edu>

To: Recipients of ABRF List <abrf@aecom.yu.edu>
cc:

Subject: RP-HPLC for mAb

Item Type: Task
Start Date: 11/07/2000 (Tuesday)
Due: 11/07/2000 (Tuesday)

We are developing RP-HPLC methods for mAbs. We've tried C18, C4, -CN and
phenyl columns from different vendors, none of them gave good results in
terms of peak shape and resolution. We saw some minor bands in SDS-PAGE and
are trying to use RP-HPLC to resolve them. Could you please share with us
your experience with RP-HPLC of mAbs or other large proteins? Thanks a lot!

Wei Zhang, Ph.D.
Genetics Institute
wxzhang@genetics.com



This archive was generated by hypermail 2b29 : Wed Nov 22 2000 - 08:37:58 EST