Dear DNAseq'ers,
we are trying to get sequences from the 3'end of cDNAs in a
medium-throughput manner on our 3700. We had been using a "T(20)VN"
primer (I mean twenty T's with (ACG)(AGCT) at the
3'-end) with some success.
But all of a sudden we got the following problem: The sequence is
more or less unreadable due to a series of T signals which disappear
after about position 200 in the read. The signal intensity of these
T-peaks varies. Also, the spacing of the T-peaks is different to that
of the "real" sequence. We do not know what we might have changed
compared to the "winning team" before ....
There are pictures of the problematic reads at:
http://adis.mpiz-koeln.mpg.de/Adis/Example-Gels/read-Tstrech-T20VN.html
Any comment is wellcome!
Thanks is advance, Bernd
-- _____________________________________________________________________PD Dr. Bernd Weisshaar MPI fuer Zuechtungsforschung (english: MPI for Plant Breeding Research) Carl-von-LinnČ-Weg 10 50829 Koeln Germany
Tel. office: +49-221-5062 590 Tel. lab: +49-221-5062 311 Fax: +49-221-5062 313 mailto:weisshaa@mpiz-koeln.mpg.de WWW-URL: http://www.mpiz-koeln.mpg.de/~weisshaa/BW-info.html
This archive was generated by hypermail 2b29 : Wed Nov 22 2000 - 08:37:58 EST