Hi y'all.
I have a couple of peptides that I'd like to have
sequenced.
Two things...
First I'd like to know of a good 'for fee' sequencing
company.
Second, my peptide has a couple of aminoisobutyric
(Aib) residues in it. What effect will that have on
the sequencing? Will the sequence simply stop there,
or will it blow through that segment and continue on
with the rest of the peptide? One of the Aib residues
is fairly close to the N-terminus.
I tip-toed through the ABRF archives and didn't find
any info about Aib with Edman sequencing (someone did
ask round 'bout June 2K, but no reply was seen).
I also did a 'Google' search on Edman Sequencing, and
Aib (aminoisobutyric acid) and didn't find anything
about the two topics together. By the way...
www.Google.com is an awesome search engine
(unsolicited praise!)
Thanks,
Steven R. Johnson B.S. Chem.
Research Associate, Process Development, Chemistry
Biomeasure, Inc. Milford, MA USA
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