Re: Hello all,

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     Hi-
     
I can't really answer your question directly, but it prompted a hair-brained
idea. If you alkylate the Cys with 3-bromopropylamine, you'll get the
aminopropylcysteine derivative. That is sufficiently like Lys (cleaved by
Lys-C,in fact) that the peptide might be well retained on a Polybrene GF/C
disk---similarly to a C-terminal Lys or Arg. Maybe that would get you to the end
without unacceptable washout.

Scott Chamberlain
Protein Structure Analysis
Chiron Corp.
510-923-2839

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Subject: Hello all,
Author: "Satya Yadav; Ph.D." <yadavs@ccf.org> at SMTP
Date: 11/13/00 11:14 AM

Hello all,
 Can anyone provide information for coupling C-terminal cysteine of a short
peptide via -SH groups to a membrane for Edman sequencing?
Thanks in advance
Satya
     
     

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