Dear Scott
You are probably best off derivatising your membrane using an appropriate hetero or homo-bifunctional crosslinker (Pierce), chosen to yield maleimides at the non-reactive end. The membrane can then be washed with PBS to remove excess cross linker. Then come in with your peptide (preincubated for a couple of hours with 5 equivs of TCEP to ensure reduced sulfhydryl) in PBS and incubate for a further hour to adhere to the membrane. A final wash to remove excess peptide andyou should be in business.
Jason
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