I was hoping that someone else would have come forward to reply with
personal experience,but I am left to give you some advice and opinions.
First,though I agree that Google is good,there is nothing like Copernic or
Web Crawler(if it is around) which combines
a lot of search engines.I tried,also,Medline,with no success.Somebody must
have tried to sequence a synthetic peptide with Aib,but must have not
thought that it was a big deal to publish.Now,to opinion.First of all there
is a PTH-Aib sold
by a number of companies.Second,Aib is actually alpha-aminoisobutyric,and
there is no reason that it would not sequence.There may be a conformation
problem,but no one has made anything out of it.Now,that I have established
that the peptide would sequence,I would say that you should do a search
of:Protein Sequencing.That should yield you
core facilities as well as commercial outfits which do the work.Maybe
someone will step forward to volunteer,otherwise
do your homework,and,hopefully,you shall be rewarded.
Steven Johnson <labswine@yahoo.com>@aecom.yu.edu> on 11/13/2000 02:28:40 PM
Sent by: Association of Biomolecular Resource Facilities
<abrf-request@aecom.yu.edu>
To: Recipients of ABRF List <abrf@aecom.yu.edu>
cc:
Subject: Edman Sequencing and Aib
Hi y'all.
I have a couple of peptides that I'd like to have
sequenced.
Two things...
First I'd like to know of a good 'for fee' sequencing
company.
Second, my peptide has a couple of aminoisobutyric
(Aib) residues in it. What effect will that have on
the sequencing? Will the sequence simply stop there,
or will it blow through that segment and continue on
with the rest of the peptide? One of the Aib residues
is fairly close to the N-terminus.
I tip-toed through the ABRF archives and didn't find
any info about Aib with Edman sequencing (someone did
ask round 'bout June 2K, but no reply was seen).
I also did a 'Google' search on Edman Sequencing, and
Aib (aminoisobutyric acid) and didn't find anything
about the two topics together. By the way...
www.Google.com is an awesome search engine
(unsolicited praise!)
Thanks,
Steven R. Johnson B.S. Chem.
Research Associate, Process Development, Chemistry
Biomeasure, Inc. Milford, MA USA
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