Re: DNAseq: polyA tails and bizzare T peaks

From: Laurence Hall (lhall@megabase.aecom.yu.edu)
Date: Tue Nov 14 2000 - 18:51:08 EST

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In reply to: Bernd Weisshaar: "DNAseq: polyA tails and bizzare T peaks"
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Dear Bernd,

I have no theoretical explanation for what you are seeing but I am pretty
confident it is an artifact of RT-PCR coupled with some sort of slippage
during cycle sequencing itself.

According to ABI, cloning your cDNA product so that you are dealing
unequivocally with a single clone invariably solves the problem.

Have you tried repeating the initial RT PCR step ??

Hope this is at least thought provoking !!

Best wishes,

Laurence Hall
******************************************************************************

Laurence Hall,
Einstein Genome Centre,
1695 Poplar Street,
New York 10461.

Tel. (00) 1 718 405 8380

Fax. (00) 1 718 405 8383

E-Mail : LHall@megabase.aecom.yu.edu

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