In one of our processing steps for a protein drug, we will be using TCEP to reduce a disulfide bond. We do have to be aware of any potential side reactions. Is anyone aware of any side reactions when proteins are treated with TCEP?
To prompt discussion, here are a couple of possibilities:
1. Incomplete reduction would produce a mixture of sulfhydryl groups and disulfide bonds, and might promote disulfide scrambling.
2. Suppose there were some methionine sulfoxide residues in the protein, due to previous oxidation. Would the reagent reduce those back to methionine?
Vernon
Vernon A. Shoup
Regeneron Pharmaceuticals
Rensselaer, NY
(518)488-6012
vernon.shoup@regpha.com
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