Hi Mary,
Maybe you could pull out the his-tagged peptide using the MC-zip-tips
charged with nickel. These tips are used for metal chelation purification
of phosphopeptides, but I don't see any reason why they couldn't be used for
a his-tagged peptide. Has anyone else out there done that before?
The peptide may not be pure enough to sequence directly, but may be enough
to get a much more simplified chromotograph and sequence from there. ???
John Hawes
Biochemistry Biotechnology Facility
Indiana University School of Medicine
www.bbf.iu.edu
-----Original Message-----
From: Mary Bower [mailto:bowerma@purdue.edu]
Sent: Friday, November 17, 2000 10:52 AM
To: Recipients of ABRF List
Subject:
Dear colleagues,
I have a protein digest which has been separated by HPLC. My customer
suspects that there is a His tag on the N-terminal end of the protein, and
would like to sequence the N-terminal fragment. Does anyone know of a
paper that refers to a technique that enables you to identify such a
peptide from a mix?
Thanks in advance.
Mary Bower
Mary Bower
Laboratory for Macromolecular Structure
Dept. Biochemistry
Purdue Unversity
West Lafayette, IN 47907
voice: (765) 494-6540
fax (765) 494-7897
This archive was generated by hypermail 2b29 : Wed Nov 22 2000 - 08:37:59 EST