Linda,
I can only comment on N-linked glycosylation. The glycosylation site does
not hinder sequencing although you will not see the ASN residue in that
cycle. Pre-treatment with N-glycanase or PGNase F will remove the
carbohydrate and modify the ASN to an ASP residue. If the glycosylation
site is high mannose (or some hybrids) and is cleaved by endoglycosidase H,
the resulting amino acid is N-acetylglucosamine asparagine who's PTH
derivative elutes between DTT and ASP-PTH. See Paxton et al, (1987)
Biochemistry 84, 920-924.
David C. Chiara, M.D., Ph.D.
Research Associate
Department of Neurobiology
Harvard Medical School
220 Longwood Avenue
Boston, Ma. 02115
ph: (617) 432-1729
fax: (617) 734-7557
Email: David_Chiara@hms.harvard.edu
www.hms.harvard.edu/bss/neuro/cohen/
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