Re: Effect of glycosylation on sequencing

• Next message: George S. Grills: "Re: In House CSA Buffer Recipe"
• Previous message: Sal Russo: "Unsubscribe"
• In reply to: Linda De Young: "Effect of glycosylation on sequencing"
• Messages sorted by: [ date ] [ thread ] [ subject ] [ author ]

At 04:44 PM 11/20/00 -0800, you wrote:
> Could someone please tell me how glycosylation effects protein
>sequencing?
>THANKS, Linda
>Linda De Young, Ph.D.
>Vice President, Product Development
>Angiogenix, Inc.

*Linda,
The short answer is the degradation will proceed past the glycosylated
residue. You'll just get a blank at the cycle with that residue. A very
good account can be found in Allen's excellent book, Sequencing of Proteins
and Peptides (1989) in the Elsevier Laboratory Techniques series (=vol 9)
(p302-09).

Regards,

John Hempel, PhD Ph (412) 624 0161
University of Pittsburgh FAX (412) 624 4759
Department of Biological Sciences
Clapp Hall 301
Pittsburgh PA 15260 USA

email: hempel@psc.edu
http://www.pitt.edu/~biology/faculty/hempel.html

• Next message: George S. Grills: "Re: In House CSA Buffer Recipe"
• Previous message: Sal Russo: "Unsubscribe"
• In reply to: Linda De Young: "Effect of glycosylation on sequencing"
• Messages sorted by: [ date ] [ thread ] [ subject ] [ author ]

This archive was generated by hypermail 2b29 : Wed Nov 22 2000 - 08:37:59 EST