Dear ABRF members,
I'm currently evaluating autosamplers to replace my old Waters WISP. I would like an autosampler with refrigeration and a very low solvent path volume. It will be used to inject peptide digests from 2D gels via a Michrom trap onto a 100 um capillary column and LCQ. Two autosamplers that come to mind are the Agilent 1100 series well plate autosampler and the LC Packings FAMOS. Does anyone have any experience with these autosamplers they would like to share, or any suggestions for other autosamplers?
The advantages of the Agilent autosampler are the price and that I already have a hand held controller to operate it. The disadvantage is that there is no software that would allow Xcalibur to control it. The advantage of the FAMOS is that it has been around longer, it can be controlled by Xcalibur, and it may be better suited for injections from very small volumes. However, are small volume injections really necessary if a trap is used to concentrate samples?
Thank you in advance for your input.
Sincerely,
Larry David
Associate Professor
Oregon Health Sciences University
davidl@ohsu.edu
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