At 11:53 22.11.00 +0100, you wrote:
>dear all,
>
>we have some problem with our abi476 which we cannot really identify at the
>moment. what we see is an extremely low initial and repetitive yield in our
>sequencing (e.g. beta-lacto tests), but the amino acid standard looks
fine. all
>valves seem to work and the injection and reagent peaks look OK. any ideas
what
>this might be??????
>
>thnx in advance for any hints,
>
>dorian
>
Hello,
we had exactly the same problem some time ago with our 473. The problem was
related to the trimethylamin. In our special case the pressurizing valve
for R2 was faulty. The valve test showed no error, but the valve did not
move (do you hear a "click" from the valve when it switches?). We changed
the vent valve (which is not really needed) with the pressurizing valve and
the intensity returned to normal values. It is difficult to detect the TMA
during the sequencing. The most convenient way is to look for
mist-formation in the waste bottle.
I hope that this could help you a bit.
Yours sincerely,
Marcus
********************************************************
Dr. Marcus Macht
University of Cologne
Centre for molecular medicine - Service laboratory
Joseph-Stelzmann-Str. 52
50931 Cologne, Germany
Tel.: +49 221 478-6995
Fax: +49 221 478-6977
e-mail: Marcus.Macht@uni-koeln.de
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