QuestionÖ
We're beginning to look into the feasibility of making
large quantitiesÖ20, 30, up to, even, 100kg/yrÖof a
couple of long peptides (30+ amino acids) that also
contain a few un-natural amino acids in the sequence.
I've seen, in trade journals and such, that there are
a couple of companies already doing so, relatively
inexpensively I might add, and am wondering how this
is done given what is available from current
technology of solid phase synthesis; solution/solid
phase fragment condensation which still requires a
significant amount of resin, solvents, etc.; or even
straight solution phase peptide assembly which has
it's own inherent problems of large solvent
requirements, and solubility issues for peptides of
this length.
Either there is something totally new (proprietary?)
or they are using vast quantities of DMF and very
large reaction vessels in addition to vast quantities
of TFA for cleavage/deprotection.
Input, replies, responses appreciated.
Regards,
Steven R. Johnson B.S. Chem
Research Associate, Process Development, Chemistry
Biomeasure, Inc. Milford, MA, USA
__________________________________________________
Do You Yahoo!?
Yahoo! Shopping - Thousands of Stores. Millions of Products.
http://shopping.yahoo.com/
This archive was generated by hypermail 2b29 : Fri Dec 08 2000 - 10:44:37 EST