nanospray LCQ Classic

From: Len Packman (lcp2@mole.bio.cam.ac.uk)
Date: Thu Nov 30 2000 - 10:15:22 EST


Dear LCQ users

In nanospray (Protana) of C8-cleaned-up in-gel digests, spraying at
0.7kV in 0.2%formic/70% MeOH I frequently see random spikes in
profile mode (largely filtered out in centroid). By collecting a
number of scans and averaging, the spikes don't really affect the
data quality but I wonder if anyone else has seen this and knows the
cause of such annoying features.

Len

-- 
*********************************************************************
Dr Len C. Packman
Assistant Director of Research
Department of Biochemistry
University of Cambridge
80 Tennis Court Road
Cambridge, CB2 1GA, UK

Tel: +44 (1223) 333639 FAX: +44 (1223) 766002 e-mail: l.c.packman@bioc.cam.ac.uk

Protein and Nucleic Acid Chemistry Facility http://www.bio.cam.ac.uk/proj/adr/PNAC/pnac.html



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