Frank,
Liang Li's lab at the Univ. of Alberta has done quite a lot of work on
polymers and MALDI.
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Gordon Alton, Ph.D.
Lead Scientist
Assay Development and Analytical Protein Chemistry
Celgene Corporation, Signal Research Division
5555 Oberlin Drive
San Diego, CA 92121
Email: galton@signalpharm.com
Phone: 858-558-7500 x8252
Fax: 858-623-0870
WWW: http://www.signalpharm.com
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-----Original Message-----
From: Frank Bruce [mailto:bfrank@valigen.net]
Sent: Thursday, November 30, 2000 1:22 PM
To: Recipients of ABRF List
Subject: MALDI Analysis of Polymers
I've seen a lot of great advice about MALDI-TOF MS of peptides, proteins and
nucleic acids being provided here so I am hoping to find a little help in
another area. We are trying to look at polymers by MALDI-TOF and have been
having some trouble getting them to fly. Our favorite test subjects are
commercially availble polyethyleneimine (2000 and 25,000 Da), polyallylamine
(15,000 and 70,000 Da) dextran (60,000-90,000 Da) and chitosan (150,000 to
540,000 Da). Now I know the highest MW compounds may be out of reach, but I
expected to be able to analyze a 2000 MW polymer. Are there any preferred
matrices, concentrations, parameters, etc. that you can send my way? Thanks
in advance for any information or citations!
Bruce.
Bruce Frank, Ph.D.
Director, Chemistry
ValiGen (formerly Kimeragen, Inc.)
300 Pheasant Run
Newtown, PA 18940
Phone: 215-504-4444 x214
Fax: 215-504-4545
email: bfrank@kimeragen.com
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