Steven,
Over coffee someone described the making of an 8 amino acid peptide at BASF in Worcester, MA.
I think their aim was to have a couple of kilos clean enough for clinical trials.
The only thing I remember is that the addition of the amino acid required a snow shovel and a lot of purification between each addition.
A 20mer at 100kg/yr? The chemistry has become more efficient but I think you need to check out your local highway department front end loader for the first additions.
But then again you can ignore this completely since you're not going to inject this into your arm. Right?
Good luck, Paul
-- ____________________________________ Paul Morrison JFB216 paul_morrison@dfci.harvard.edu Molecular Biology Core Facilities Dana-Farber Cancer Institute http://mbcf.dfci.harvard.edu 44 Binney Street 617-632-3082 Boston, MA 02115 fax 632-4814 ____________________________________Steven Johnson <labswine@yahoo.com> wrote: >QuestionÖ > >We're beginning to look into the feasibility of making >large quantitiesÖ20, 30, up to, even, 100kg/yrÖof a >couple of long peptides (30+ amino acids) that also >contain a few un-natural amino acids in the sequence. > >I've seen, in trade journals and such, that there are >a couple of companies already doing so, relatively >inexpensively I might add, and am wondering how this >is done given what is available from current >technology of solid phase synthesis; solution/solid >phase fragment condensation which still requires a >significant amount of resin, solvents, etc.; or even >straight solution phase peptide assembly which has >it's own inherent problems of large solvent >requirements, and solubility issues for peptides of >this length. > >Either there is something totally new (proprietary?) >or they are using vast quantities of DMF and very >large reaction vessels in addition to vast quantities >of TFA for cleavage/deprotection. > >Input, replies, responses appreciated. > >Regards, >Steven R. Johnson B.S. Chem >Research Associate, Process Development, Chemistry >Biomeasure, Inc. Milford, MA, USA > > >__________________________________________________ >Do You Yahoo!? >Yahoo! Shopping - Thousands of Stores. Millions of Products. >http://shopping.yahoo.com/ >
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