Hi
Anyone interested in large scale synthesis of either peptides or oligonucleotides should check out the Tides 2001 meeting scheduled for April 24-27 in Tuczon, AZ. See
www.ibc-lifesci.com for info.
Paul Morrison wrote:
> Steven,
>
> Over coffee someone described the making of an 8 amino acid peptide at BASF in Worcester, MA.
> I think their aim was to have a couple of kilos clean enough for clinical trials.
> The only thing I remember is that the addition of the amino acid required a snow shovel and a lot of purification between each addition.
>
> A 20mer at 100kg/yr? The chemistry has become more efficient but I think you need to check out your local highway department front end loader for the first additions.
>
> But then again you can ignore this completely since you're not going to inject this into your arm. Right?
>
> Good luck, Paul
>
> --
> ____________________________________
> Paul Morrison JFB216 paul_morrison@dfci.harvard.edu
> Molecular Biology Core Facilities
> Dana-Farber Cancer Institute http://mbcf.dfci.harvard.edu
> 44 Binney Street 617-632-3082
> Boston, MA 02115 fax 632-4814
> ____________________________________
>
> Steven Johnson <labswine@yahoo.com> wrote:
> >QuestionÖ
> >
> >We're beginning to look into the feasibility of making
> >large quantitiesÖ20, 30, up to, even, 100kg/yrÖof a
> >couple of long peptides (30+ amino acids) that also
> >contain a few un-natural amino acids in the sequence.
> >
> >I've seen, in trade journals and such, that there are
> >a couple of companies already doing so, relatively
> >inexpensively I might add, and am wondering how this
> >is done given what is available from current
> >technology of solid phase synthesis; solution/solid
> >phase fragment condensation which still requires a
> >significant amount of resin, solvents, etc.; or even
> >straight solution phase peptide assembly which has
> >it's own inherent problems of large solvent
> >requirements, and solubility issues for peptides of
> >this length.
> >
> >Either there is something totally new (proprietary?)
> >or they are using vast quantities of DMF and very
> >large reaction vessels in addition to vast quantities
> >of TFA for cleavage/deprotection.
> >
> >Input, replies, responses appreciated.
> >
> >Regards,
> >Steven R. Johnson B.S. Chem
> >Research Associate, Process Development, Chemistry
> >Biomeasure, Inc. Milford, MA, USA
> >
> >
> >__________________________________________________
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> >http://shopping.yahoo.com/
> >
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