Has anyone else tried to avoid buying NED-labeled oligos from ABI by substituting TAMRA-labeled oligos? We're using the filter set D with a ROX size standard and the FAM, HEX and NED dyes for our microsattelite markers. We just did a quick experiment on our 3100 and found that the TAMRA was detected as yellow peaks just like NED! Like the other dyes, there was a small amount of leakage into the other colours when the PCR product was over-loaded, but nothing that will interfere significantly with pooling multiple markers We still have to compare the same marker amplified with both TAMRA and NED dyes to see how their mobilities and intensities compare (i.e. if the alleles are labeled as the same size), but that shouldn't prevent us from ordering new markers in the TAMRA dye rather than the NED. Just thought this might interest anyone genotyping or synthesizing labeled oligos.
Marcia
Marcia MacDonald
Xenon Genetics, Inc.
100-2386 East Mall
Vancouver, BC
Canada V6T 1Z3
604-221-8423 fax
This archive was generated by hypermail 2b29 : Fri Dec 08 2000 - 10:44:37 EST