It was nice to see Daniel Wellner's reference to Persson's survey on the
types of residues most often N-acetylated. Is anyone aware of anything
similar on phosphorylated residues? I've heard 1% for tyrosine in the
past, and I suppose serine is at least 65%, but if anyone can point me to
the existence of a survey on this I would be most grateful.
Regards,
John
At 03:01 PM 12/4/00 -0500, Daniel Wellner wrote:
>........There was a paper by J.L. Brown and W.K. Roberts (1976),
>J.Biol.Chem. 251:1009-1014, in which they found that approximately 80% of
>the soluble proteins in Ehrlich ascites cells (and probably other
>eukaryotic cells) are acetylated at their N-terminal residues.
>
> Another paper which may be relevant is a survey of 250 proteins
>with N-terminal acetylation by Persson et al. (Eur.J.Biochem. (1985),
>152:523-527). They looked at the N-terminal sequence of 105 of these
>proteins and found that the most frequently acetylated residue was serine
>(43/105), followed by alanine (33/105).
>
> You may be interested in a method which is available for
>selectively removing the acetyl group from an N-terminal N-acetylserine (or
>N-acetylthreonine). This method allows the blocked protein to be sequenced
>by Edman degradation. (D. Wellner et al.(1990), Proc.Nat.Acad.Sci.USA
>87:1947-1949).
>
> Best wishes,
>
> Daniel
>
>Daniel Wellner, Ph.D.
>Department of Biochemistry
>Weill Medical College of Cornell University
>New York, NY 10021
>
John Hempel, PhD Ph (412) 624 0161
University of Pittsburgh FAX (412) 624 4759
Department of Biological Sciences
Clapp Hall 301
Pittsburgh PA 15260 USA
email: hempel@psc.edu
http://www.pitt.edu/~biology/faculty/hempel.html
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