>dear abrf,
>
>a while ago some people posted a protocol for producing neutravidin chips to
>replace biacore%s sa-chips. we did the neutravidin immobilisation
>according to
>this protocol and we do indeed get a surface capable of binding biotinylated
>peptides. but, different from biacore%s chips the immobilized peptide is
>washed
>off the chip during regeneration! is this normal and if not, does anyone
>know a
>reason for this? we have couple of thousand RUs on the surface.
>
>best regards,
>
>dorian
Hi Dorian,
We noticed this problem, too. We have changed our neutravidin protocol
recently. We now regenerate the neutravidin chips after coupling with
three one minute injections of 0.1% SDS. After binding of analytes, we
regenerate with 0.05% SDS. Baseline is stable under these conditions.
This applies to protein peptide interactions. We also always use the extra
cleanup function when performing regeneration. Hope this helps.
Chris
***********************************
Christian R. Lombardo, PhD
Director, Molecular Analysis Facility
The Burnham Institute
10901 North Torrey Pines Road
La Jolla, CA 92037
(858) 646-3100 x3627
crl@burnham-inst.org
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