>Hi Bruce - Here's a few options. When using volatile solvents,
>multiple spotting of a uL of the analyte on the target plate helps.
>Please let me know if you get a decent matrix for chitosan. Regards-
\Jim Kerwin
Mass Spectrometry Facility
Cornell University
Ithaca, NY 14853
Tel 607-254-4848
>
>
>At 16.21 30/11/00 -0500, you wrote:
>>I've seen a lot of great advice about MALDI-TOF MS of peptides, proteins
and
>>nucleic acids being provided here so I am hoping to find a little help in
>>another area. We are trying to look at polymers by MALDI-TOF and have
been
>>having some trouble getting them to fly. Our favorite test subjects are
>>commercially availble polyethyleneimine (2000 and 25,000 Da),
polyallylamine
>>(15,000 and 70,000 Da) dextran (60,000-90,000 Da) and chitosan (150,000
to
>>540,000 Da). Now I know the highest MW compounds may be out of reach, but
I
>>expected to be able to analyze a 2000 MW polymer. Are there any preferred
>>matrices, concentrations, parameters, etc. that you can send my way?
Thanks
>>in advance for any information or citations!
>>
>>Bruce.
>>
>>Bruce Frank, Ph.D.
>>Director, Chemistry
>>ValiGen (formerly Kimeragen, Inc.)
>>300 Pheasant Run
>>Newtown, PA 18940
>>Phone: 215-504-4444 x214
>>Fax: 215-504-4545
>>email: bfrank@kimeragen.com
>>
>>
>Prof. Piero Pucci
>Dipartimento di Chimica Organica e Biochimica
>Universitý di Napoli Federico II
>Complesso Universitario Monte S. Angelo
>via Cinthia - 80126 Napoli
>Tel. +39-081-674318; Fax: +39-081-674313
>e
>Centro Internazionale Servizi di Spettrometria di Massa
>via Pansini 5 - 80131 Napoli
>http://sco.technapoli.it/mass/Home.html
>Tel. +39-81-5465136 Fax +39-81-5465484
______________________________________________________________________
This e-mail is confidential to the intended recipient. If you have received
it in error you must not copy, disclose, distribute or rely on its contents
but delete it immediately from your computer and notify BPL please by
telephoning +44 (0) 20 8258 2589.
BPL is not responsible and cannot be held liable
-for any reason to anyone who has received this e-mail in error
-for the contents of personal or private messages sent by members of staff
-for the security of information sent by e-mail at your request
______________________________________________________________________
This e-mail is confidential to the intended recipient. If you have received
it in error you must not copy, disclose, distribute or rely on its contents
but delete it immediately from your computer and notify BPL please by
telephoning +44 (0) 20 8258 2589.
BPL is not responsible and cannot be held liable
-for any reason to anyone who has received this e-mail in error
-for the contents of personal or private messages sent by members of staff
-for the security of information sent by e-mail at your request
This archive was generated by hypermail 2b29 : Fri Dec 08 2000 - 10:44:37 EST