In a message dated 12/7/00 9:37:08 PM Pacific Standard Time,
luojun@er6s1.eng.ohio-state.edu writes:
Is there any other common software? If you are current using this kind
of software, could you kindly give me some suggestion? Also can you tell
me what kind of equipment you are using to import the gel to computer?
Can I use normally scanner to do that?
Jun Luo
luo.32@osu.edu
-----------------------------------
Dear Luojun:
www.AlphaInnotech.com lists powerful 2D software; so does www.cgen.com. I
have had demo software from AlphaInnotech, but not yet evaluated Compugen.
You can also send a 2D image for analysis, and get a report back. For this
beginning, you could use a good office desktop scanner, but you should get a
better scanner for your 2D work.
2D scanners should be of the professional quality like BioRad and Pharmacia,
and must have a transparency adapter to measure through stained gels. Now
these can be purchased on the internet instead of the private labels they
offer--good savings, and then get the 2D software you like. Generally 300
or 600 dpi will give you the maximum information; 1200 dpi just seems to fill
up file space and takes longer to analyze. But the present professional
scanners from UMAX, AGFA, EPSON will produce good images and cost about
$2000-$3000. Maybe less this week.
You can also use the high performance fluorescence/visible gel documentation
imagers from Alpha Innotech for analyzing 2D gels or blots with multiple
fluor labels. Their AlphaMatch 2D software will overlay and match multiple
gel images easily.
A good test for any software is to take the same gel and move it or rotate it
on the scanner, then analyze the two images. At what accuracy will the
software find and quantify the spots from the two images? This should be
below 1% variation.
Walter Schick
BioSepCo
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