Recently someone has brought me a gel band which is stained very dark with
coomassie.They want the mass of the intact protein.I extracted the protein
according to the paper of Cohen and Chait (Ana. Biochem.
1997,247,257-267)using formic acid:water:isopropanol(1:3:2) but get a very
weak and broad signal.I suspect that it is the high concentration of coomassie
that is interfering.Does anybody know of a procedure that will reduce the
amount of coomassie in the sample.Thanks.
sincerly,
Paul Semchuk
Institute for Biomolecular Design
University of Alberta
Edmonton,Alberta,Canada
This archive was generated by hypermail 2b29 : Fri Jan 05 2001 - 08:42:46 EST