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> -----Original Message-----
> From: pds [SMTP:pds@fossil.biochem.ualberta.ca]
> Sent: Friday, December 08, 2000 9:44 AM
> To: Recipients of ABRF List
> Subject: sds page band
>
> Recently someone has brought me a gel band which is stained very dark with
>
> coomassie.They want the mass of the intact protein.I extracted the protein
>
> according to the paper of Cohen and Chait (Ana. Biochem.
> 1997,247,257-267)using formic acid:water:isopropanol(1:3:2) but get a very
>
> weak and broad signal.I suspect that it is the high concentration of
> coomassie
> that is interfering.Does anybody know of a procedure that will reduce the
> amount of coomassie in the sample.Thanks.
>
> sincerly,
> Paul Semchuk
> Institute for Biomolecular Design
> University of Alberta
> Edmonton,Alberta,Canada
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