MJ Basestation Read Length

From: Jeremy Medalle (medallj@mail.rockefeller.edu)
Date: Sun Dec 10 2000 - 18:35:15 EST

Next message: Ken Mitchelhill: "RE: phosphopeptides/mass spec"
Previous message: VictorVDV2@aol.com: "Queations about an Homemade X-ray"
In reply to: Sheryl Christofferson: "Re: MJ Basestation"
Next in thread: Jennifer Brosius: "RE: MJ research DNA Sequencer(Basestation)"
Messages sorted by: [ date ] [ thread ] [ subject ] [ author ]

Hi Sheryl,

Christopher Abbot, MJ tech, ran our samples for ~3 hrs. We got ~400-600
usable bps using 30 cm well-to-read plates. (Our samples were backup
samples that had sat in the freezer for about 2 weeks.)

Depending on glass plates and gel run, you can get the following according
to their brochure:

Collection Time(hrs) Read Length(bps)
   1.5 450-650
   3.5 650-850
   5.0 850-1000+

-- 
Jeremy Medalle
Lab Coordinator
The Rockefeller University
1240 York Ave
Box 105
NY, NY 10021
http://www.protein13-pc.rockefeller.edu
> From: Sheryl Christofferson <sherylc@omrf.ouhsc.edu>
> Organization: Oklahoma Medical Research Foundation
> Date: Sat, 09 Dec 2000 10:47:48 -0600
> To: Recipients of ABRF List <abrf@aecom.yu.edu>
> Cc: Recipients of ABRF List <abrf@aecom.yu.edu>
> Subject: Re: MJ Basestation
> 
> 
> 
> Hi Jeremy:
> 
> This machine sounds very nice. One detail that you neglected to mention (or I
> neglected to see) is the length of read. How many bases can you get in 5
> hours?
> 
> 
> 
> Sheryl Christofferson
> ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
> Oklahoma Medical Research Foundation
> DNA Sequencing Facility
> Oklahoma City, Oklahoma
> (405) 271-7393 ext. 34565
> www.omrf.ouhsc.edu/OMRF/Core/DNASEQ/
> sherylc@omrf.ouhsc.edu
> 
> 
>

Next message: Ken Mitchelhill: "RE: phosphopeptides/mass spec"
Previous message: VictorVDV2@aol.com: "Queations about an Homemade X-ray"
In reply to: Sheryl Christofferson: "Re: MJ Basestation"
Next in thread: Jennifer Brosius: "RE: MJ research DNA Sequencer(Basestation)"
Messages sorted by: [ date ] [ thread ] [ subject ] [ author ]

This archive was generated by hypermail 2b29 : Fri Jan 05 2001 - 08:42:46 EST