Chaps buffer for membrane protein extraction

From: Jose Cesar Rosa (jrosa@cisunix.unh.edu)
Date: Tue Dec 12 2000 - 20:30:03 EST


Dear ABRFers.
Is someone out there has an idea how much efficient is CHAPS buffer (35mM
Tris-HCl pH 8.0, 8M urea, 65mM DTT and 4% CHAPS) for membrane protein
extraction? Is there other detergent which could be more efficient than CHAPS?
Thanks in advance for any information on this matter.
Best regards,
Cesar
Jose Cesar Rosa PhD
Dept. of Chemistry
University of New Hampshire
23 College rd. Parsons Hall
Durham NH 03824
phone: (603) 862-3797



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