Terry,
Electrospray mass spectrometry can be applied to analyzing oligos if run in
negative ion mode. The key is to thoroughly dialyze out the metal cations.
For example, check:
"Characterization of oligonucleotides and nucleic acids by mass spectrometry",
Limbach,P.A.; Crain,P.F.; McCloskey,J.A. Curr.Opin.Biotechnol.6, 96-102 (1995).
"Negative electrospray ionization mass spectrometry of synthetic and chemically
modified oligonucleotides"
Potier,N.; van Dorsselaer,A.; Cordier,Y.; Roch,O.; Bischoff,R., Nucleic Acids
Research 22, 3895 (1994)
Hope this helps,
Russ Lehrman
|--------+----------------------->
| | "Terry |
| | Stoming" |
| | <TSTOMING@MAI|
| | L.MCG.EDU> |
| | |
| | 12/13/2000 |
| | 09:32 AM |
| | |
|--------+----------------------->
>--------------------------------------------------------|
| |
| To: Recipients of ABRF List |
| <abrf@aecom.yu.edu> |
| cc: (bcc: Russ Lehrman/Inhale) |
| Subject: Oligo quality control |
>--------------------------------------------------------|
Does anyone have a method for ms using an API 100 electrospray mass spec to
determine the quality(mol.wt) of synthetic oligos?
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