I just did a small literature search on "Fmoc deprotection" and came up
with this:
Wade JD, Mathieu MN, Macris M and Tregear, GW
Letters in Peptide Science 7:(2) 107-112 Mar 2000
The authors looked at a variety of bases, with a view toward inhibiting
aspartimide formation as well as getting efficient deprotection. While
nothing worked as well as HMB protection, they found that a solution of
Piperazine containing 0.1 M HOBt worked better than piperidine or DBU.
Piperazine (diethylenediamine) is available from the various Sigma-Aldrich
companies at a price comparable to piperidine. I don't think piperazine
is a controlled substance.
Hope this helps.
Angela C. Murphy
On Wed, 13 Dec 2000, Sergey V. Burov wrote:
> Dear All,
>
> Presumably, it is a question regard to Russian peptide chemists or may
> be somebody has similar problems. During last several months we try
> to order piperidine from different chemical companies (I donít know any
> Russian vendors). Unfortunately all our attempts were unsuccessful.
> There are strong limitation from our custom and it is necessary to have
> the permission
> from special committee. One year ago there were several possibilities to
> buy
> this reagent, but not now. I know it is possible to substitute
> piperidine by DBU
> or other chemicals, but I donít like this way (as for the application of
> DBU, it commonly also takes a small amount of piperidine). So, there are
> real
> problems for our Fmoc chemistry. Any ideas or experience in this field?
>
> With best regards,
> Sergey Burov
>
> Institute of Macromolecular Compounds,
> 199004, V.O., Bolshoi pr. 31,
> St.-Petersburg, Russia
>
> Tel: 7 (812) 323 36 07
> Fax: 7 (812) 328 68 69
> Email: burov@hq.macro.ru
> diapharm@diapharm.spb.ru
>
*******************************************
Angela C. Murphy, Chemist
Lab. of Cell Biology, NHLBI, NIH
3 Center Drive, MSC 0301, Rm. B1-22
9000 Rockville Pike
Bethesda, MD 20892-0301 USA
tel.: (301) 496-2324
fax: (301) 402-1519
email: acmurphy@helix.nih.gov
*******************************************
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