Re: Misc, SPS-PAGE, sensitive protein stain needed

From: Jill Hendrickson (JillHendrickson@probes.com)
Date: Thu Dec 14 2000 - 13:10:37 EST

Although it's nice for image analysis, you don't need a laser scanner to use
SYPRO Ruby stain. You can also see it with a simple UV transilluminator and
document with Polaroid film. If you use a good transilluminator with 15 watt
bulbs and the appropriate filter, you can get great sensitivity. We provide an
inexpensive filter for Polaroid cameras.

http://www.probes.com/servlets/product?region=USA&item=6656
http://www.probes.com/servlets/product?region=USA&item=12000 

--
Jill Hendrickson, Ph.D.
Molecular Biology Product Manager
Molecular Probes, Inc.
phone 541-465-8356
fax  541-465-4593
jill@probes.com

Ken Mitchelhill wrote:

> Norman,
>
> Sypro Ruby is probably the most sensitive. It is sold by either Molecular
> Probes (see http://www.probes.com/lit/feature/syprodyes/section1.html) or
> BioRad under licence but you will neeed a flourescent scanner . There is
> some disagreement in the literature but I believe silver is about the same,
> especially if you use a fixing protocol including formaldehyde or
> glutaraldehyde. Of course silver is technically a bit more complex but you
> can use a visible scanner. If you just want to visualize, then there are
> quite a few kits on the market for this type of stain or it's pretty simple
> to make up your own reagents. There is a good protocol at
> http://www.fhcrc.org/labs/hahn/methods/biochem_meth/silver_stain.html
>
> Caveat: if you want to do any downstream processing (sequencing or mass
> spec) don't use this type of silver stain. Use either Sypro Ruby or read
> about silver staining for mass spec in the excellent ASMS poster of Ejvind
> Moertz et. al. downloadable at http://www.protana.com/publics/default.asp
>
> Good luck...Ken
>
> --
> Ken Mitchelhill PhD
> Principal Instrumentation Scientist
> Vision Instruments Limited
> Private Bag 18, 495 Blackburn Road
> Mount Waverley  3149  Victoria  AUSTRALIA
> Voice: 61 3 9211 7428 Fax: 61 3 9211 7401
> Ken.Mitchelhill@visioninst.com
> http://www.visioninst.com
>
> > -----Original Message-----
> > From: Norman Watts [mailto:WATTS@calvin.niams.nih.gov]
> > Sent: Thursday, December 14, 2000 12:15 AM
> > To: Recipients of ABRF List
> > Subject: Misc, SPS-PAGE, sensitive protein stain needed
> >
> >
> > Hi all,
> >
> > What is currently considered to be the most sensitive and
> > dependable stain for detecting protein bands on SDS-PAGE
> > gels? I expect ca. 0.05 ug/band.
> >
> > norman
> >
> > -------------------------------------------------------
> >
> > Norman Watts, PhD.
> > Lab. of Structural Biology
> > Building 6, Room B2-34
> > 6 Center Drive MSC2717
> > NIAMS, National Institutes of Health
> > Bethesda, MD 20892-2717
> > U.S.A.
> > E-mail:       watts@calvin.niams.nih.gov
> > Phone:        301-402-3418
> > Fax:  301-480-7629
> >
> > -------------------------------------------------------
> >

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