Dear ABRF:
This is a question for these guys with experience in sequencing
deglycosilated proteins.I treated a reduced plus 4-VP protein, with PGNase.I
used the vendor suggested conditions. For " desalt " the mixture, I used a
short C8 column, eluting with the usual 0.1% TFA-Acetonitrile buffers.At the
supposed N-glycosilated position, I found a small Asn signal, plus a smaller
peak, between DTT and Asp ( perhaps Iso-Asp ? ). As far as I know, I would
wait only Asp there. Any suggestions ?
Thanks in advance for your input.
Susana L.
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Susana Linskens
LANAIS-PRO
Junin 956
1113 Buenos Aires - ARGENTINA
linskens@qb.ffyb.uba.ar
FAX: 54-11-4508-3652
Phone: 54-11-4508-3651
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