MS: Tryptic Digest Stability

From: Derek Bradley (Derek.Bradley@ucl.ac.uk)
Date: Mon Dec 18 2000 - 16:19:43 EST


Hi Guys,

Does anyone out there know how stable tryptic digest solutions are? I'm
generally only able to do nanospray MS/MS analysis on samples after they're
at least 2-3 days old and often older (stored at -20C) and tend to find
great difficulty in getting any meaningful results from them. I realise that
immediate analysis would be preferable but this isn't always possible so
what is the best way of storing them ? Currently due to processing methods
they are stored in 10% formic acid solution at -20C.

Does anyone have any ideas?

Cheers,

Derek Bradley
Dept. of Medicine
UCL



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