Sequencing PCR products

From: Karyn T O'neil (Karyn.T.ONeil@dupontpharma.com)
Date: Wed Dec 20 2000 - 10:37:08 EST

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Does anyone have a recommendation for sequencing PCR products? We find
that if the sequencing reactions are precipitated with isopropanol we have
a significant problem with dye blobs. When we use columns to clean up the
unincorporated dyes, we end up losing a significant amount of the signal.
We have only tried columns from EDGE and I am wondering if anyone has
experience with a column where you do not lose so much DNA but the dye
blobs are effectively removed.

Karyn O'Neil

Sr. Research Scientist
DuPont Pharma. Co.
Applied Biotechnology Dept.
P.O. Box 80336
Wilmington, DE 19880-0366

phone: 302-695-1227
fax: 302-695-9420

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