Dear Derek -
We've not had any major problems with the kinds of losses you're
experiencing. We've (Peter Backlund and I) stored our peptides for a
year or more in 1:1 ACN::0.1% TFA. As of yesterday we got a MALDI
spectrum of such a sample that gave a really super spectrum.
Al and Peter
Hi Guys,
Does anyone out there know how stable tryptic digest solutions are? I'm
generally only able to do nanospray MS/MS analysis on samples after they're
at least 2-3 days old and often older (stored at -20C) and tend to find
great difficulty in getting any meaningful results from them. I realise that
immediate analysis would be preferable but this isn't always possible so
what is the best way of storing them ? Currently due to processing methods
they are stored in 10% formic acid solution at -20C.
Does anyone have any ideas?
Cheers,
Derek Bradley
Dept. of Medicine
UCL
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