Misc.: Scanners vs. Digital Cameras for Capturing Gel Images

From: Bleibaum, Janice {Mass~Palo Alto} (JANICE.BLEIBAUM@roche.com)
Date: Wed Jan 03 2001 - 19:40:20 EST


> Dear List,
>
> We would like to know if anyone has tried using a digital camera for
> capturing gel images. We have been using a flatbed scanner equipped to
> handle transparent images (transmissive light rather than merely
> reflective light) but not all the images captured with the scanner have
> been of the quality we would like. Specifically, we see spots in our gels
> that the scanner does not always record. If you are using a digital
> camera, we would like answers to the following questions:
>
> Which model?
> If you switched from a scanner, why did you switch? How much improvement
> in image quality do you see?
> What stain are you using? (We've been using colloidal Coomassie blue.)
> What format are using for the captured image? (We've been recording
> images as .tif files from the scanner.)
> Do you import the captured image into a gel analysis program such as
> Melanie or Imagemaster? Any problems with file formats?
>
> Any other comments which come to mind on this issue are welcome.
>
> Sincerely,
>
> Janice Bleibaum
>
> Janice Bleibaum
> Roche Bioscience
> Protein Sequencing and Proteomics
> 3401 Hillview Ave.
> M/S S3-1
> Palo Alto CA 94304
> (650)852-1639
> janice.bleibaum@roche.com
>



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