DNA sequencing problems

From: kathyi@cgl.ucsf.edu
Date: Mon Jan 08 2001 - 20:38:30 EST

Next message: William H. Vensel: "LCQ and .dta files"
Previous message: John Stults: "ABRF 2001 advance registration deadline soon"
Next in thread: Bayliss, Stuart: "RE: DNA sequencing problems"
Maybe reply: Bayliss, Stuart: "RE: DNA sequencing problems"
Messages sorted by: [ date ] [ thread ] [ subject ] [ author ]

Is anyone having problems or successes sequencing a modified bluescript
containing a yeast expression vector plus insert on an ABI 3700. One user
reports that his templates work on a ABI 310, but that the same sample give
very broad peakson our ABI 3700, indicative of some degree of capillary
clogging on our 3700. Both runs are with Big Dye chemistry.

Thanks for your input/suggestions.

Kathy

Kathryn Ivanetich, Ph.D.
Acting Director
Biomolecular Resource Center, UCSF
Box 0541, San Francisco, CA 94143
Ph: 415 514-0101x4
FAX: 415 476-7974
email: kathyi@cgl.ucsf.edu

BRC Web page: http://www.ucsf.edu/brc

Next message: William H. Vensel: "LCQ and .dta files"
Previous message: John Stults: "ABRF 2001 advance registration deadline soon"
Next in thread: Bayliss, Stuart: "RE: DNA sequencing problems"
Maybe reply: Bayliss, Stuart: "RE: DNA sequencing problems"
Messages sorted by: [ date ] [ thread ] [ subject ] [ author ]

This archive was generated by hypermail 2b29 : Thu Feb 01 2001 - 12:44:47 EST