Hello all,
Here is a puzzler for you. Did some sequencing for a client who only
wanted 5 cycles, and had two pieces of PVDF--we were to sequence them
separately. There were threonines in cycles two and five of both
samples. Sequence was identical for both samples. (sorry, I can't
give it publicly without a lot of checking). In cycle two of sample
two, the threonine yield was reduced about 50%, with a corresponding
rise in all of the byproduct peaks. Cycle five threonine of the same
sample gave the usual amount of threonine. In sample one, cycles two
and five were usual yields for threonine, with the usual amount of
byproducts.
Does anyone know why this would be, or even have a theory? I could
explain both threonines in one sample being low, but I can't come up
with any rationale to explain this.
Thanks for your insight!
-Weather inconsequential: Beautiful break in the winter yesterday
and today. Sunny and in the high 40's. I got to go to my son's
elementary school and work as recess volunteer yesterday. I actually
got to stand around in the sunshine in the middle of the day!!! I
need to have recess too I think.
-laurey
Laurey Steinke
Protein Structure Core Facility
University of Nebraska Medical Center
Omaha Nebraska, 68198-4525
Phone (402) 559-6647
FAX (402) 559-6650
http://www.unmc.edu/pscf/
lsteinke@molbio.unmc.edu
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