Janice,
On our Bio-Rad GS-700 scanner, it is essential to use a gray-Plexiglas diffuser
plate to get high quality images of wet gels. Without the diffuser plate, we
see spots in the Coomassie-stained gels that aren't on the image, just as you
described. Conversely, with the diffuser plate placed on the wet gel, on the
computerized image we see spots that we can't visualize well enough on the gel
to cut them out by hand. Perhaps if you used such a plate, you would get
satisfactory images from your flatbed scanner.
Gary
Gary Davis
Bayer Corp.
400 Morgan Lane
West Haven, CT 06516
E-mail: gary.davis.b@bayer.com
"Bleibaum, Janice {Mass~Palo Alto}" <JANICE.BLEIBAUM@roche.com>@aecom.yu.edu> on
01/03/2001 07:40:20 PM
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Subject: Misc.: Scanners vs. Digital Cameras for Capturing Gel Images
> Dear List,
>
> We would like to know if anyone has tried using a digital camera for
> capturing gel images. We have been using a flatbed scanner equipped to
> handle transparent images (transmissive light rather than merely
> reflective light) but not all the images captured with the scanner have
> been of the quality we would like. Specifically, we see spots in our gels
> that the scanner does not always record. If you are using a digital
> camera, we would like answers to the following questions:
>
> Which model?
> If you switched from a scanner, why did you switch? How much improvement
> in image quality do you see?
> What stain are you using? (We've been using colloidal Coomassie blue.)
> What format are using for the captured image? (We've been recording
> images as .tif files from the scanner.)
> Do you import the captured image into a gel analysis program such as
> Melanie or Imagemaster? Any problems with file formats?
>
> Any other comments which come to mind on this issue are welcome.
>
> Sincerely,
>
> Janice Bleibaum
>
> Janice Bleibaum
> Roche Bioscience
> Protein Sequencing and Proteomics
> 3401 Hillview Ave.
> M/S S3-1
> Palo Alto CA 94304
> (650)852-1639
> janice.bleibaum@roche.com
>
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