Laurey,
What about partial phosphorylation at the cycle two threonine in sample 2?
Linda
Big Apple Minutes: We won't have a Giants/Jets superbowl but one out of two is not bad if the Giants
win this weekend.
With little bits of intermittent snow after the storm which dumped a foot, the city has a pleasantly
clean appearance.
Linda Siconolfi-Baez
Laboratory for Macromolecular Analysis
Albert Einstein College of Medicine
Ullman 405
1300 Morris Park Avenue
Bronx, N.Y.10461
718-430-2864
lsiconol@aecom.yu.edu
http://www.bioc.aecom.yu.edu/labs/angellab/
----------
>From: Laurey Steinke <lsteinke@molbio.unmc.edu>
>To: Recipients of ABRF List <abrf@aecom.yu.edu>
>Subject: ProtSeq: Threonine degradation
>Date: Tue, Jan 9, 2001, 1:43 PM
>
>Hello all,
>Here is a puzzler for you. Did some sequencing for a client who only
>wanted 5 cycles, and had two pieces of PVDF--we were to sequence them
>separately. There were threonines in cycles two and five of both
>samples. Sequence was identical for both samples. (sorry, I can't
>give it publicly without a lot of checking). In cycle two of sample
>two, the threonine yield was reduced about 50%, with a corresponding
>rise in all of the byproduct peaks. Cycle five threonine of the same
>sample gave the usual amount of threonine. In sample one, cycles two
>and five were usual yields for threonine, with the usual amount of
>byproducts.
>
>Does anyone know why this would be, or even have a theory? I could
>explain both threonines in one sample being low, but I can't come up
>with any rationale to explain this.
>
>Thanks for your insight!
>
>
>-Weather inconsequential: Beautiful break in the winter yesterday
>and today. Sunny and in the high 40's. I got to go to my son's
>elementary school and work as recess volunteer yesterday. I actually
>got to stand around in the sunshine in the middle of the day!!! I
>need to have recess too I think.
>
>-laurey
>Laurey Steinke
>Protein Structure Core Facility
>University of Nebraska Medical Center
>Omaha Nebraska, 68198-4525
>
>Phone (402) 559-6647
>FAX (402) 559-6650
>http://www.unmc.edu/pscf/
>lsteinke@molbio.unmc.edu
>
>
>
>
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