Laurey,
I could come up with the esoteric:
Alternative splicing in one of the two copies of the message because of some
heterozygosity in the strain in which the protein was purified. (If your
animal is diploid).
Or the mundane:
A solenoid is going bad in one of your blocks giving you intermittent
deliveries.
Get the same result three more times and at least you get to cross out the
solenoid.
Or even more mundane:
E. coli contamination from any of the 49 ORF's from that organism that start
that way. (list appended below). Random chance would put the orf's in e.coli
starting with this pattern at 10.7 (For example MTxT appears 12 times) so
the MTxxT pattern is over represented.
All of these are wrong of course. (At least I wouldn't bet a 6 pack on this
bs). I'm just too excited to sleep after seeing the titanium powerbook
today.
Steve Jobs is the man. :-)
Trust me, nothing usable here,
Paul
____________________________________
Paul Morrisonİİ JFB216İİİİİİİİİİİİİİ paul_morrison@dfci.harvard.edu
Molecular Biology Core Facilitiesİİİ
Dana-Farber Cancer Instituteİİİİİİİİ http://mbcf.dfci.harvard.edu
44 Binney Streetİİİİİİİİİİİİİİİİİİİİ 617-632-3082
Boston, MAİ 02115İİİİİİİİİİİİİİİİİİİ fax 632-4814
____________________________________
49 E. coli ORF's starting with MTxxT
MTAKT
MTALT
MTCST
MTCYT
MTDKTI
MTDKTS
MTDNT
MTDVT
MTEFT
MTEHT
MTESTS
MTESTT
MTGIT
MTGMT
MTHMT
MTHMT
MTHMT
MTHMT
MTHMT
MTHST
MTIST
MTIYT
MTLET
MTLFT
MTLPT
MTLRT
MTMIT
MTMPT
MTMRTG
MTMRTN
MTNFT
MTNITK
MTNITL
MTNLT
MTQFT
MTQLT
MTTET
MTTLTA
MTTLTL
MTTLTV
MTTNTH
MTTNTV
MTTQTQH
MTTQTQY
MTTQTV
MTTVT
MTVPT
MTVQT
-----Original Message-----
From: Laurey Steinke
To: Recipients of ABRF List
Sent: 1/9/01 1:43 PM
Subject: ProtSeq: Threonine degradation
Hello all,
Here is a puzzler for you. Did some sequencing for a client who only
wanted 5 cycles, and had two pieces of PVDF--we were to sequence them
separately. There were threonines in cycles two and five of both
samples. Sequence was identical for both samples. (sorry, I can't
give it publicly without a lot of checking). In cycle two of sample
two, the threonine yield was reduced about 50%, with a corresponding
rise in all of the byproduct peaks. Cycle five threonine of the same
sample gave the usual amount of threonine. In sample one, cycles two
and five were usual yields for threonine, with the usual amount of
byproducts.
Does anyone know why this would be, or even have a theory? I could
explain both threonines in one sample being low, but I can't come up
with any rationale to explain this.
Thanks for your insight!
-Weather inconsequential: Beautiful break in the winter yesterday
and today. Sunny and in the high 40's. I got to go to my son's
elementary school and work as recess volunteer yesterday. I actually
got to stand around in the sunshine in the middle of the day!!! I
need to have recess too I think.
-laurey
Laurey Steinke
Protein Structure Core Facility
University of Nebraska Medical Center
Omaha Nebraska, 68198-4525
Phone (402) 559-6647
FAX (402) 559-6650
http://www.unmc.edu/pscf/
lsteinke@molbio.unmc.edu
This archive was generated by hypermail 2b29 : Thu Feb 01 2001 - 12:44:47 EST