Laurey,
I had a similar loss of Threonine. Is one of the Threonines partially
Phosphorylated? Did you see a peak eluting ever so slightly before
Asp? What else have you heard about this?
Michael E. Ellis
Laurey Steinke wrote:
> Hello all,
> Here is a puzzler for you. Did some sequencing for a client who only
> wanted 5 cycles, and had two pieces of PVDF--we were to sequence them
> separately. There were threonines in cycles two and five of both
> samples. Sequence was identical for both samples. (sorry, I can't
> give it publicly without a lot of checking). In cycle two of sample
> two, the threonine yield was reduced about 50%, with a corresponding
> rise in all of the byproduct peaks. Cycle five threonine of the same
> sample gave the usual amount of threonine. In sample one, cycles two
> and five were usual yields for threonine, with the usual amount of
> byproducts.
>
> Does anyone know why this would be, or even have a theory? I could
> explain both threonines in one sample being low, but I can't come up
> with any rationale to explain this.
>
> Thanks for your insight!
>
> -Weather inconsequential: Beautiful break in the winter yesterday
> and today. Sunny and in the high 40's. I got to go to my son's
> elementary school and work as recess volunteer yesterday. I actually
> got to stand around in the sunshine in the middle of the day!!! I
> need to have recess too I think.
>
> -laurey
> Laurey Steinke
> Protein Structure Core Facility
> University of Nebraska Medical Center
> Omaha Nebraska, 68198-4525
>
> Phone (402) 559-6647
> FAX (402) 559-6650
> http://www.unmc.edu/pscf/
> lsteinke@molbio.unmc.edu
-- Michael E. Ellis Colorado State University Macromolecular Resources Dept of Biochemistry, 355 MRB Ft. Collins, CO 80523
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