Bob,
Quite simple actually...make your peptide as usual.
On the N-terminus add either Myristic acid as a normal
aminoacid type coupling, or you can use the more
expensive (and somewhat wasteful) myristic anhydride
and add it like you'd do for capping or acetylating.
If you want to put it internally into the sequence,
like on a lysine, use Lys(Dde) and put your N-terminal
aminoacid on as the Boc version (assuming you're doing
FMOC chemistry) then treat the peptide with 2%
hydrazine in DMF (2 x 10min, same volume as your
depro. sol'n) to remove the Dde group from the lysine,
wash, and then add the myristic group in either
fashion as above. Normal TFA cleavage will remove the
N-terminal Boc group.
Hope this helps.
Regards,
Steven Johnson, B.S. Chem.
Research Associate
Process Development, Chemistry
Biomeasure, Inc. Milford, MA
--- boblee <boblee@uic.edu> wrote:
> Hi everyone,
>
> Need protocol for synthesize the myristoylated
> peptide. Thanks.
>
> Bob Lee
>
>
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