Hello all,
I recall maybe a year ago someone commenting that Glu-C does not work well
in in-gel digests since it is 50kDa whereas proteases like Lys-C are ~25kDa
and can penetrate gel pores more easily. That made sense. But, just
looking now at the product sheet on Glu-C, it is listed as 30kDa, and the
sequence given, ~250aa, is consistent with that or a little less.
The question is, does anyone know if the functional enzyme is a dimer?
That would rationalize all the above.
Thanks,
John
John Hempel, PhD Ph (412) 624 0161
University of Pittsburgh FAX (412) 624 4759
Department of Biological Sciences
Clapp Hall 301
Pittsburgh PA 15260 USA
email: hempel@psc.edu
http://www.pitt.edu/~biology/faculty/hempel.html
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