Happy 3rd millennium, as well as new year, to all!
Although the yield does seem low to me, too, this could be an
appropriate yield if any unusual additions were in the sequence, i.e.
fluorescent, biotin, CY3, 8-0x0-dG, thiol, amino,etc.
I do run into low yields when lower coupling efficiency monomers are in
the sequence.
Another comparison I would do is to look at the range of yield
expectations the company lists for 0.2 umole scale and see what the
percentage is on yield received after purification by HPLC.
Hope this helps. By the way, if this involves an unusual monomers in
the sequence, I will gladly discuss this with the customer and see if
our facility can help. If they only need small amts of full length
product, our prices will probably be too high to warrant the use of us.
take care
Winnell
Scottie Adams wrote:
>
> Happy New Year to all!
>
> One of my users ordered a 15 mer from a vendor. They ordered the
> 0.2 umole scale and asked that it be HPLC purified. They received 93 ug.
> ( micrograms)
>
> This seems quite low to me, but since I have been out of the DNA synthesis
> business for a while, maybe my memories are a little "rose-colored".
>
> Any comments would be appreciated. Thank you.
>
> Scottie
>
> Scottie Adams aka Pamela Scott Adams
>
> Manager
> Molecular Biology Core Facility
> Trudeau Institute
> 100 Algonquin Avenue
> Saranac Lake, NY 12983
> Phone: 518-891-3080, Ext. 115
> Fax: 518-891-5126
> Email: sadams@northnet.org
> sadams@trudeauinstitute.org
> http://www.trudeauinstitute.org
-- Winnell H. Newman, Director, Nucleic Acids Facility (NAF) North Carolina State University, Department of Molecular and Structural Biochemistry 128 Polk Hall, Box 7622 Raleigh, NC 27695-7622Nucleic Acids Facility Web Page http://www.ncsu.edu/naf Email = mailto:winnell@ncsu.edu VOX (919) 515-3463 FAX (919) 515-2047
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