Hello,
I want to identify the site of ADP-ribosylation in a certain protein by
LC-MS/MS. We have got a Q-TOF2 hybrid MS. The amino acid to be
ADP-ribosylated should be an arginine. Is there anyone around having
experience with identification of ADP-ribosylated peptides by LC-MS?
Are there techniques to enrich the ribosylated peptide (like IMAC for
phosphopeptides) or are there known neutral losses which can be used
(like 98 for phosphoserine/phosphothreonine) to screen for the peptide?
Is the ADP-ribose stable during fragmentation?
I know this are a lot of questions but till know I was not successful in
finding literature concerning this topic.
Any help would be appreciated.
Thanks
Markus
Markus Piotrowski
Ruhr-Universitaet Bochum
Dep. Plant Physiology, ND3
44801 Bochum
Germany
Markus.Piotrowski@ruhr-uni-bochum.de
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