Martin,
We were looking at a 3100 but went with MJ Research BaseStation. When we
analyzed the cost (instrument + labor + reaction cost/sample), we saw that
the 3100 would cost between $4-$5/ sample while the BaseStation was about
$2. Basically we will pay for our instrument in savings in the first year
(Mostly due to the chemistry savings and no expensive matrix).
Additionally, we can run many different templates and reaction conditions
with minimal optimization and get great data. The noise is so low it is
incredible. Plus we are now getting 800bp average runs! The people I know
using the 3100 replace the capillaries every 500 runs or so which get's
quite expensive...also they get about 550bp averages (Phred 20) if they are
lucky. I hope this helps.
Chris
>From: Martin Pentony <MPent@sbri.org>
>To: Recipients of ABRF List <abrf@aecom.yu.edu>
>Subject: DNA Seq:request for 3100 opinions and running costs
>Date: Wed, 17 Jan 2001 15:11:55 -0800
>
>We will probably be getting a 3100 sequencer. I was hoping to hear some
>opinions on the machine and if there are any possible problems unique to
>the machine particularly with the life of the capillaries since they
>cannot be regenerated? Also we are reviewing the pricing for our DNA
>sequencing service and I was wondering if people have some information
>on their running costs. I am just getting involved in the dollar
>problems and it would be a great help to be able to compare to other
>peoples numbers. Many thanks
>Martin Pentony
>
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