Dr. Seow,
You might want to read through the following:
Voss, T., Habert, P., "Observations on the reproducibility and matching
efficiency of two-dimensional electrophoresis gels: Consequences for
comprehensive data analysis, Electrophoresis, 2000, 21, 3345-3350.
Best regards,
Lloyd W. Sumner, Ph.D.
Assoc. Scientist & Head, Biological Mass Spectrometry
The Samuel Roberts Noble Foundation
PO Box 2180
2510 Sam Noble Parkway
Ardmore, OK 73402
Voice 580-221-7392
Fax 580-221-7380
Email lwsumner@Noble.Org
-----Original Message-----
From: Seow Teck Keong [mailto:teckeong@nus.edu.sg]
Sent: Friday, January 19, 2001 12:08 AM
To: Recipients of ABRF List
Subject: P2D: Reproducibility of 2-DE Gels
Hello
I am just wondering if anyone out there has seen any paper or done any work
to establish the reproducibility, in terms of percentage, of 2-DE gels. I
know there are many papers out there claiming that it is no longer a
problem, especially with the introduction of IPG strips, but has anyone
given any hard figures on this?
Hope to hear it from the 2-DE gurus out there.
Regards,
Teck Keong SEOW, PhD
Research Fellow
Bioprocessing Technology Centre
National University of Singapore
10 Medical Drive
Singapore 117597
Tel: +65-8745120
Fax: +65-7754933
Email: teckeong@nus.edu.sg
Web Site: http://www.eng.nus.edu.sg/btc/ <http://www.eng.nus.edu.sg/btc/>
ICQ: 25510422
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