Antw: P2D: Reproducibility of 2-DE Gels

From: Marcus Macht (mma@bsax.de)
Date: Fri Jan 19 2001 - 09:49:40 EST


There was recently an interesting article in Electrophoresis concerning this topic. The title was "Observations on the reproducibility and matching efficiency of two-dimensional electrophoresis gels: Consequences for comprehensive data analysis" by Tilman Voss and Peter Haberl in Volume 21, Issue 16, 2000.
Hope that helps.

Yours sincerely,
Marcus

--------------------------------------------------------------
Dr. Marcus Macht
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Proteomics

Bruker Saxonia Analytik GmbH
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email: mma@bsax.de

>>> Seow Teck Keong <teckeong@nus.edu.sg> 19.01.2001 07.08 Uhr >>>
Hello
 
I am just wondering if anyone out there has seen any paper or done any work
to establish the reproducibility, in terms of percentage, of 2-DE gels. I
know there are many papers out there claiming that it is no longer a
problem, especially with the introduction of IPG strips, but has anyone
given any hard figures on this?
 
Hope to hear it from the 2-DE gurus out there.
 
Regards,
 
Teck Keong SEOW, PhD
Research Fellow
Bioprocessing Technology Centre
National University of Singapore
10 Medical Drive
Singapore 117597
Tel: +65-8745120
Fax: +65-7754933
Email: teckeong@nus.edu.sg
Web Site: http://www.eng.nus.edu.sg/btc/ <http://www.eng.nus.edu.sg/btc/>
ICQ: 25510422
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