Dear colleagues,
I have a researcher asking for a separation of tiny amounts of DNA oligomers (25-mer, 50-mer and 55-mer) in one fraction from the 80bp hybridization product. The hybridization product has to be clean for further ligation experiments. Because of very low concentration of the product I am hesitating to use a gel filtration. Does anyone have any experience using ultrafiltration spin-columns (such as the Amicon or the Eppendorf sell) for nucleic acids. I am especially concerned about non-specific membrane sorption (like some proteins do...). Thanks for your input.
Hana
Hana Konecna
Core Facility
Laboratory of Functional Genomics and Proteomics
Faculty of Science, Masaryk University
Kotlarska 2, 611 37 Brno, Czech Republic
hanak@sci.muni.cz
http://www.sci.muni.cz/LMFR/
Phone 420-5-411 29 369
FAX 420-5-411 29 372
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